Enzymatic and spectral analysis of cytochrome oxidase in adult and fetal rat liver and Morris hepatoma 3924A.

chrome oxidase content of tumors, both types of assay methods have not been used concurrently. Experiments described in this communication were designed to provide information about the cytochrome oxidase content of Morris hepatoma 3924A relative to normal, host, and fetal liver by using both the oxygen electrode enzymatic assay (jolarographic assay) and the spectral assay. As will be described below, both assay methods are compatible and clearly show that the cytochrome oxidase content is signifi cantly reduced in Morris hepatoma 3924A relative to control liver. In addition, experiments described below indicate that the cytochrome oxidase activity of fetal liver cells is very close to that of the 3924A hepatoma.